Bach2 12014
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| Gene ID: | 12014 |
| Gene symbol: | bach2 |
| Gene Description: | btb and cnc homology 2 |
| Aliases: | MGC117590 |
| Human Ortholog Gene ID: | [60468] |
| Reviewer Judgement: | TF Gene |
| Functional Classification: | DNA-Binding: sequence-specific |
| DBD Protein Group Classification: | Zipper-Type Group |
| DBD Protein Group/Family Classification: | Zipper-Type Group / Leucine Zipper Family |
| Homolog Cluster(s): | 107503 |
| Pubmed ID | Function | Species | Evidence Strength | Reviewer's Comments | Reviewer |
| [8887638] | DNA Binding; Transactivation | Mouse | Strong | Bach2 demonstrates binding as a Bach2-MafK heteromdimer to the chicken beta-globin NF-E2 response elemement (5'-TCGACCCGAAAGGAGCTGACTCATGCTAGCCC-3') in gel shift assays.
Binding site selection studies performed using a bacterially synthesized MBP-Bach2 fusion protein identified a Bach2 consensus binding site (cgTGAGTCAtc). Transient transfection of quail QT6 fibroblasts and chicken erythroid HD3 cells show that Bach2 represses transcription mediated by a reporter plasmid containing NF-E2 sites from the chicken beta-globin 3' enhancer driving luciferase expression. | Rob Sladek |
| [10949928] | DNA Binding | Human | Strong | Sequence-specific DNA binding to the IgH HS3 (5'-GCTGAGGGCAGCTGAGTCATCCTGAGCCT-3') was demonstrated in BACH2-transfected RAJI cells by antibody supershift in a gel-shift assay. | Rob Sladek |
| Additional Reviewer Comments: |
Reviewer: Rob Sladek Bach2 demonstrates binding as a Bach2-MafK heteromdimer to the chicken beta-globin NF-E2 response elemement (5'-TCGACCCGAAAGGAGCTGACTCATGCTAGCCC-3') in gel shift assays. Binding site selection studies performed using a bacterially synthesized MBP-Bach2 fusion protein identified a Bach2 consensus binding site (cgTGAGTCAtc). Transient transfection of quail QT6 fibroblasts and chicken erythroid HD3 cells show that Bach2 represses transcription mediated by a reporter plasmid containing NF-E2 sites from the chicken beta-globin 3' enhancer driving luciferase expression. |
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| Gene description | btb and cnc homology 2 + |
| Gene id | 12,014 + |
| Gene judgment | TF Gene + |
| Gene symbol | bach2 +, and MGC117590 + |
| Gene taxonomy | DNA-Binding: sequence-specific + |
| Homolog cluster | 107,503 + |
| Protein family | 3 Zipper-Type Group/21 Leucine Zipper Family + |
| Protein group | 3 Zipper-Type Group + |
