Atr 245000
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| Gene ID: | 245000 |
| Gene symbol: | atr |
| Gene Description: | ataxia telangiectasia and rad3 related |
| Aliases: | |
| Human Ortholog Gene ID: | [545] |
| Reviewer Judgement: | TF Gene Candidate |
| Functional Classification: | Transcription Regulatory Activity: heterochromatin interaction/binding;
Single stranded RNA/DNA binding; Transcription Factor Binding: tf co-factor binding |
| Homolog Cluster(s): | 245000 |
| Pubmed ID | Function | Species | Evidence Strength | Reviewer's Comments | Reviewer |
| [10545197] | Other | Human | Strong | Human ATR immunoprecipitate was significantly enriched for CHD4, HDAC2, MTA1, and MTA2 when ATR was immunoprecipitated from HeLa cell extracts using conjugated anti-ATR beads. CHD4, HDAC2, MTA1, and MTA2 were detected by Western blot. | Magdalena Swanson |
| [12011431] | DNA Binding | Human | Medium | In a pull-down assay, ATR was shown to preferentially bind UV-damaged DNA over unmodified DNA when human Flag-tagged ATR was purified from HEK293T cells and incubated with labelled UV (6-4) photoproduct DNA or the unmodified 46bp oligomer. In vitro kinase assays carried out in the presence or absence of UV-damaged DNA showed that recombinant human ATR can phosphorylate recombinant human p53 and that the phosphorylation of p53 by purified ATR is enhanced by damaged DNA. | Magdalena Swanson |
| Additional Reviewer Comments: |
Reviewer: Magdalena Swanson Atr is a TF gene candidate. The endogenous human protein was found to coprecipitate with members of the nucleosome remodeling and deacetylating complex, namely CHD4, HDAC2, MTA1, and MTA2, in immunoprecipitation studies. Recombinant human ATR was shown to preferentially bind UV-damaged DNA in pull-down assays. Kinase assays showed that the recombinant protein can phosphorylate recombinant human p53, and that this phosphorylation is enhanced by damaged DNA. Current literature does not prove a transcription function for Atr, but rather indicates that Atr is involved in DNA repair. Seems to be an ssDNA damage binding protein (PMID 12011431). WWW |
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| Gene description | ataxia telangiectasia and rad3 related + |
| Gene id | 245,000 + |
| Gene judgment | TF Gene Candidate + |
| Gene symbol | atr + |
| Gene taxonomy | Transcription Regulatory Activity: heterochromatin interaction/binding +, Single stranded RNA/DNA binding +, and Transcription Factor Binding: tf co-factor binding + |
| Homolog cluster | 245,000 + |
